The Dan bus drivers' union began a strike on Monday morning on all of the company's lines that connect Tel Aviv to Bat Yam.
The strike includes around 250 bus routes, and is meant to last until Monday evening, Army Radio reported.
The drivers were demanding pay increases and improvements in work conditions, threatening more strikes if no solution is found.
Dan responded to the strike, saying "we are dealing with an unnecessary strike; the Dan company is now in a period of cut-backs." The company was going to hire pensioners to step in so that bus lines could continue running.
The routes through Bat Yam that will be halted include: 6, 8, 10, 18, 19, 25, 26, 40, 42, 43, 44, 46, 140, 143, and 243.