Israel will reopen the Karni cargo crossing between Gaza and Israel on Thursday morning, security officials said.
Israeli officials first closed the crossing in January, and kept it closed for most of the last month, citing security threats warning of Palestinian attacks. Palestinian officials subsequently discovered a tunnel nearby and said they sealed it.
Palestinians believe the closure is retribution for Hamas' victory in January legislative elections.
Boosting cargo traffic through Karni was a key aspect of an agreement brokered by US Secretary of State Condoleezza Rice last November, an accord meant to give momentum to peace efforts after Israel's summer pullout from the Gaza Strip.