A Palestinian assailant attempted to stab a soldier at the Beit Anoun junction on Route 60, north of Kiryat Arab in the Hebron area on Sunday.
According to the IDF, the attempted attack occurred during Palestinian riots in the area.
No Israelis were wounded in the attack.
The suspected terrorist was shot and killed by security forces, the IDF stated.
The incident was the latest in a string of attempted stabbing attacks against security forces in the Hebron area.
On Saturday, ten Palestinian rioters were shot and injured by IDF Ruger rounds during a violent clash in the city.
An army spokeswoman said some 100 Palestinians launched a violent disturbance at the Hassam Shoter army position in the West Bank city, hurling rocks, Molotov cocktails, burning tires, and firing marbles from sling shots.
The IDF responded with Ruger rounds, aiming at the legs of rioters.
The condition of those wounded was not known to the army.
Earlier, thousands of Palestinians took part in funerals of terrorists killed in attacks on Israelis after Israel returned their bodies to the Palestinian Authority in Hebron.Yaakov Lappin contributed to this report.