Separately, Kuwait condemned what it described as drone attacks by Iran and its proxies targeting vital facilities in the country, calling the strikes a violation of its sovereignty.
The reopening of the Strait, which carries about a fifth of the world's oil consumption, is an important component of the US-Iran ceasefire deal.
Pakistan's Defense Minister Khawaja Asif's comments came as Islamabad positions itself as a major player in ceasefire talks between the US, Israel, and Iran.
The agreement prioritizes the railway sector, including a proposal to link Anatolia in the north with the port of Aqaba in the south and extend toward the Gulf.
"Iran is not seeking war but will not forfeit its rights and considers all resistance fronts as a unified entity," Khamenei added.
IRAN AFFAIRS: The Islamic Republic has spent 47 years trying to root itself inside America, building a network of institutions designed to shape influence in Washington along the regime’s ideals.
Former US CENTCOM Chief Gen. Kenneth "Frank" McKenzie Jr. spoke to The Jerusalem Post on Thursday regarding issues surrounding the war with Iran.
Ending the war prematurely “could embolden the Iranian authorities, making them more repressive and self-assured,” warned Kako Aliyar, a member of a Kurdish Iranian opposition party.
Iran's deputy foreign minister, Saeed Khatibzadeh, said that despite reports to the contrary, the Strait of Hormuz remains open to civilian ships that coordinate with Iranian authorities.
Israel bombed more targets in Lebanon on Thursday after launching its largest attacks of the war on Wednesday.
"This war will be remembered as Trump's grave strategic miscalculation. One whose consequences reshaped the region in unintended ways," Middle East scholar Fawaz Gerges told Reuters.