"Pakistan is a major non-NATO ally of the United States. That will remain the case, but we would urge restraint in this case," a State Department spokesman said.
The series of attacks threaten to escalate tensions between the countries and escalate tensions in South Asia.
Iran said on Tuesday it had targeted Israel-linked militant bases inside Pakistan.
The foreign ministry said Iran's strike on targets in Pakistani terrritory was an unprovoked and blatant breach of Pakistan's sovereignty and was "unacceptable."
Iran's Revolutionary Guard attacked targets in Pakistan just one day after shelling Iraq and Syria with missiles.
Pakistan and Afghanistan are the only countries where the virus is still endemic, and in recent years more than 200 polio vaccination workers have been killed.
This resolution turns the tide in the country by igniting a new chapter in the dialogue on justice and crime prevention.
Islamabad dismisses the ruling as invalid, sparking a new chapter in the long-standing Kashmir dispute.
The bomb and gun attack occurred in the district of Dera Ismail Khan on the edge of the lawless tribal regions bordering Afghanistan, the two security officials said.
Six of the nine people murdered have since been identified. A total of 26 others were left injured from the attack.
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