The novel coronavirus is believed to have originated in a wet market in Wuhan and first emerged in December before spreading quickly worldwide. Some 2.83 million people have been reported to be infected globally and 197,872 have died, according to a Reuters tally.
"The latest news is that by April 26, the number of new coronavirus patients in Wuhan was at zero, thanks to the joint efforts of Wuhan and medical staff from around the country," National Health Commission spokesman Mi Feng said at a briefing.
The city had reported 46,452 cases, 56% of the national total. It saw 3,869 fatalities, or 84% of China's total.
Wuhan and the province of Hubei were put in lockdown near the end of January, with roads sealed, trains and planes canceled and residents unable to move freely for more than two months. The city is still testing residents regularly despite relaxing the restrictions.
The focus has since shifted to the northeast border province of Heilongjiang, which has seen large numbers of imported coronavirus cases entering from Russia.
China's health authority had earlier reported 11 new coronavirus cases on the mainland on April 25, down from 12 the previous day, with no fatalities.