RECIFE, Brazil - With the knockout rounds of the World Cup coinciding with the beginning of Ramadan, Muslim players must decide whether to observe the month-long religious fast which begins this weekend.France, Germany, Switzerland, Belgium, Algeria and Nigeria are among the teams that have Muslim players who may choose to observe the 30-day period of fasting and reflection.During Ramadan, the ninth and holiest month in the Islamic calendar, all healthy adult Muslims are expected to refrain from eating and drinking during daylight hours.Such a scenario could play havoc with the tightly-controlled diets of elite professional athletes, especially in the hot, humid conditions in which some World Cup games in Brazil are being played."The challenge is mainly trying to maintain hydration on a daily basis, and secondly trying to maintain energy levels," Emma Gardner, performance nutritionist at the English Institute of Sport, told Reuters."Muscle mass is also an issue. Research suggests that people can lose muscle mass through the period of Ramadan, although it tends to be during the early period," she said.Gardner's comments appear to be at odds with those of Jiri Dvorak, FIFA's chief medical officer, who told a media briefing on Monday that players observing the fast should not suffer any deterioration in their physical condition."We have made extensive studies of players during Ramadan, and the conclusion was that if Ramadan is followed appropriately, there will be no reduction in the physical performances of players," Dvorak told reporters.OZIL SAYS CANNOT FASTAll healthy adult Muslims are expected to observe the month-long fast, although exceptions can be made and the fast postponed.One player who has made up his mind is Germany's Mesut Ozil."Ramadan starts on Saturday, but I will not take part because I am working," he told a press conference on Wednesday.Nutritionist Gardner has previously worked with a player from the British hockey team who fasted during a major tournament. She describes her experience with the athlete as being "a case study" in how to handle the issue."For players observing the fast during the World Cup, the optimal timing for games would be evening kickoffs, as it's closest to the time when they can replenish and rehydrate," she said, explaining strategies to offset the impact of fasting."Some athletes choose to use mouth rinsing, where they basically rinse their mouth our with water but don't ingest any," said Gardner, who has previously worked with English soccer club Blackburn Rovers."There is research to show that there are cognitive benefits, it makes them feel like they're drinking. Otherwise you can use things like cold towels to help them cool down."Dehydration can have a negative effect on performance, with a loss of one to two percent of body fluids by weight leading to problems with concentration.However, the nutritionist said that the best way to help fasting players was to consult with their coaches to ensure that their physical activity and recovery were properly planned, recommending they train only once a day."Ideally, from looking into research, the best time for athletes who are partaking in Ramadan to train is either early in the morning or late at night, as near to a time when they can hydrate and eat."Refueling the body under such circumstances is not easy, as a whole day's intake has to be made up during the hours of darkness."I found at the break of the fast, to get back to a hydrated state the athlete had to drink around six liters of fluid," Gardner said. "Standard guidelines say two and a half to three liters of fluid a day, so many people are surprised when they hear six."On its own, that amount of water can have a diuretic effect, so we had to make sure there was some sodium or that it was consumed with food."