An illustrative picture of the Neuronal Positional System used for brain mapping.
(photo credit: DR. SHLOMO TSURIEL AND DR. ALEX BINSHTOK)
Throughout our waking lives, we are exposed to a continuous stream of stimuli and experiences, some of which trigger the strengthening of connections between neurons in the brain, and begin the process of forming memories. However, these initial memory traces are fragile and only a small number will become longterm memories with the potential to last a lifetime. For this transition to occur, the brain must stabilize the memory traces through a process called consolidation.
During this process, the brain produces new proteins that strengthen the fragile memory traces. However, if a new experience occurs while an existing memory trace is being consolidated, the new stimuli could disrupt or even hijack the consolidation process.
The brain partially solves this problem by postponing some of the memory consolidation to a period in which new experiences are minimalized, that is, while we are asleep. But what happens if we wake up while consolidation is taking place? How does the brain prevent events that occur just after awakening from interrupting the consolidation process? Bar-Ilan University scientists show that a protein synthesis-dependent process blocks new learning just after waking, thereby preventing the disruption of the memory stabilization that occurs during sleep.
A new study by Prof. Abraham Susswein, of BIU’s Mina and Everard Goodman Faculty of Life Sciences and The Leslie and Susan Gonda (Goldschmied) Multidisciplinary Brain Research Center has now answered this question. Recently published in eLife, the article’s first author is Roi Levy, whose doctoral research – conducted in Susswein’s lab – is described in the present study, which also includes part of the doctoral research of David Levitan.
Susswein and his colleagues have used a seemingly unlikely subject for their study, namely the marine slugs called “sea hares” or Aplysia. These marine slugs are convenient for neuroscientific investigation because of their simple nervous systems and large neuron and because they have been shown to be capable of basic forms of learning.
Just after training during waking hours, proteins are synthesized to initiate the consolidation of new memory. Consolidation proteins are produced again in greater quantities during sleep for subsequent processes on the memory trace. The researchers found that blocking the production of consolidation proteins in sleeping sea slugs prevents these creatures from forming long-term memories, confirming that, like us, they do consolidate memories during sleep.
The researchers now show that exposing sea slugs to new stimuli immediately after they wake up does not trigger the formation of new memories. In a learning paradigm affecting sea slugs’ feeding activity, the animals were trained after being awakened from sleep. On awakening, interactions between new experiences and consolidation are prevented because the brain blocks long-term memory arising from the new stimuli. However, when the researchers treated the slugs just prior to the training with a drug that inhibits protein production, they found that the new stimuli could generate long-term memory. These findings show that proteins blocking the formation of new memories prevent an experience upon waking from being effective in producing memory. Removing this block – by inhibiting protein production – allows experiences just after waking to be encoded in memory.
This even applies to experiences that are too brief to trigger memory formation in fully awake sea slugs.
“The major insight from this research is that there is an active process in the brain which inhibits the ability to learn new things and protects the consolidation of memories,” Susswein explained.
The researchers also compared learning by fully- awake sea slugs trained in isolation and those trained with companions. They discovered that training in social isolation appears to inhibit new learning, and identified similar molecular processes common to both training in isolation and to training on waking from sleep.
“Our next step following on from this work,” said Susswein, “is to identify these memory-blocking proteins and to fathom how they prevent the formation of new memories. We may also find that the blocking process accounts for why we cannot remember our dreams when we wake up.”