Tel Aviv University.
(photo credit: WIKIMEDIA)
A breakthrough technology from Tel Aviv University makes it possible to manipulate pathogens by delivering DNA into drug-resistant bacteria.
The discovery is a blow to antimicrobial resistance, one of the biggest threats to global health that can affect anyone, at any age, in any country. Some 700,000 people in the world die each year, on average, due to a failure of antibiotics to kill resistant bacteria. The figure, according to the World Health Organization, could rise to 10 million per year by 2050.
The research expands the range of bacteriophages as the primary tool for introducing DNA into pathogenic bacteria to neutralize their lethal activity. A single type of bacteriophage can be adapted to a wide range of bacteria, an innovation that is likely to accelerate the development of potential drugs.
Prof. Udi Qimron of the department of clinical microbiology and immunology at the university’s Sackler Faculty of Medicine led the research team, which also included Dr. Ido Yosef, Dr. Moran Goren, Rea Globus and Shahar Molshanski- Mor, all of Qimron’s lab. The study was recently published in the journal Molecular Cell and featured on its cover.
For the research, the team genetically engineered bacteriophages to contain the desired DNA, rather than their own genome. They also designed combinations of nanoparticles from different bacteriophages, resulting in hybrids that are able to recognize new bacteria, including pathogenic bacteria.
The researchers also used directed evolution to select hybrid particles able to transfer DNA with optimal efficiency.
DNA manipulation of pathogens includes sensitization to antibiotics, Qimron said. “We’ve developed a technology that significantly expands DNA delivery into bacterial pathogens. This may indeed be a milestone, because it opens up many opportunities for DNA manipulations of bacteria that were impossible to accomplish before.”
He added that this process could pave the way to change the human microbiome – the combined genetic material of the microorganisms in humans – by replacing virulent bacteria with non-virulent bacteria and replacing antibiotic-resistant bacteria with antibiotic-sensitive bacteria, as well as changing environmental pathogens.
The team applied for a patent on this technology and is developing products that would use this technology to deliver DNA into bacterial pathogens, making them not virulent and causing them to be sensitive to antibiotics, Qimron said.
The research has been granted $700,000 from the Momentum Fund, a unique $24 million fund established by Ramot, Tel Aviv University’s Business Engagement Center. This funding enables the advanced development of this technology within the university’s laboratories.
The Momentum Fund invests in promising breakthrough technologies in a wide range of fields, including pharmaceuticals, healthcare, high-tech and the physical sciences. Tata Group is the lead investor of the Momentum Fund. Singapore-based Temasek is another key investor.