Flies mating (illustrative).
(photo credit: INGIMAGE)
“Birds do it, bees do it, even educated fleas do it; let’s do it, let’s fall in love,” went the old Cole Porter song. Ninety years later, Bar-Ilan University researchers have found that male fruit flies find sex – and more specifically ejaculation of their sperm – to be an inherently rewarding experience.
The study, just published in the journal Current Biology, is the first to show that the rewarding nature of ejaculation is found among animals, from fruit flies to mammals. It also adds to evidence that manipulating sexual experience in the flies affects their interest in consuming alcohol.
“Successful mating is naturally rewarding to male flies and increases the levels of a small peptide in the brain called neuropeptide F,” said Dr. Galit Shohat-Ophir of Bar-Ilan University’s Mina and Everard Goodman Faculty of Life Sciences and Gonda (Goldschmied) Multidisciplinary Brain Research Center. “Male flies that are sexually deprived have increased motivation to consume alcohol as an alternative reward.”
Shohat-Ophir, along with the study’s lead author Shir Zer-Krispil and their colleagues, came to these conclusions by taking advantage of optogenetic tools. Optogenetics (from the Greek words for “visible” and “origin”) is a biological technique in which light is used to control cells in living tissue, typically neurons, that have been genetically modified to express light-sensitive ion channels. It utilizes a combination of techniques from optics and genetics to control and monitor the activities of individual neurons in living tissue – even within freely-moving animals – and to measure these manipulation effects exactly and in real-time.
For their research, they used fruit flies in which neurons expressing the neuropeptide corazonin (CRZ) could be switched “on” by exposure to red light. Earlier evidence shows that CRZ neurons in the fly’s abdomen trigger the release of sperm and seminal fluid. By examining flies in this way, the researchers were able to explore the rewards associated with ejaculation apart from other aspects of a sexual encounter.
“We wanted to know which part of the mating process entails the rewarding value for flies,” Shohat-Ophir explained. She suggested that it could be the actions that males perform during courtship, female pheromones or the last step of mating in which sperm and seminal fluid are released.
The study was supported by the Israel Science Foundation and Marie Curie Career Integration Grants.
TO FIND OUT whether ejaculation produced an immediate pleasurable response, the researchers used an arena in which one side emitted a red light to trigger optogenetic activation of CRZ neurons and tracked where male flies chose to spend their time. Those experiments showed that flies showed a strong preference for the red light, implying that ejaculation in itself is a rewarding experience.
Next, they trained the flies to associate the red light and ejaculation with a particular odor. They then tested whether the flies preferred the scent that reminded them of that past experience of ejaculation; in fact, it did.
After a few days of repeated activation of CRZ neurons, the male fruit flies had high levels of neuropeptide F in their brains, similar to males that actually mated with female partners. When the first group of males was given the choice between ordinary liquid food and liquid food spiked with alcohol, they preferred the non-alcoholic food. In contrast, control flies and engineered males not exposed to red light preferred the alcohol.
“The principles by which the brain processes reward are extremely conserved in all animals; this is a really basic everyday machinery that helps animals survive,” Shohat-Ophir said. “Drugs of abuse use the same systems in the brain that are used to process natural rewards. This allows us to use simple model organisms to study aspects of drug addiction, including the interplay between natural and drug rewards and the connection between experience and the mechanisms that underlie the risk to develop drug addiction.”
The researchers plan to continue the investigation of how information about ejaculation or successful mating reaches the brain. They also want to use their findings to further understand an individual’s risk for developing an addiction, noting that addiction risk is influenced by a combination of molecular and neuronal mechanisms that influence the likelihood of progressing from initial drug exposure to repeated use.
“Our studies suggest that the state of the animal – undergoing successful mating or being rejected – affects the motivation to consume drug rewards,” said Shohat-Ophir. “An analogy for reward states can be proposed in which a high-reward state is illustrated by a full ‘reservoir’ and low state by an empty ‘reservoir.’ One can speculate that vulnerability to addiction is related to the size of the ‘reservoir’ that needs to be filled, or to different sensitivity to changes in the reward levels.”
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