The 'Al-Awda' boat was carrying dozens of international activists and two Israeli citizens.
"Then the Palestinians can live in peace and rebuild the land that once was theirs."
The petition leading to the court order was filed on behalf of the Gavish family, who was killed in the 2002 Passover terrorist attack at the Park Hotel in Netanya.
This is the second time in the past two months that Palestinians from Gaza have sent a flotilla into the Mediterranean in an attempt to breach the naval blockade.
A spokesman said that “The flotilla will set out at 11 a.m. carrying sick and injured Palestinians who have been unable to travel abroad.”
The flotilla marks eight years since Israeli naval commandos raided the Turkish ship MV Mavi Marmara.
Before this year's Gaza flotilla left Gothenburg, a solidarity ceremony was held in the Swedish city with the participation of hundreds of left-wing activists from Scandinavia and Europe.
Australian premier, NZ governor-general to arrive for Beersheba battle commemoration.
Some of the relatives expressed anger that the deal was not discussed with them first.
Dozens of pro-Palestinian activists gathered outside the courthouse ahead of the court session waving Palestinian flags and placards.