Afghan President Hamid Karzai lashed out at the United States on Friday for failing to cooperate fully with an investigation into the massacre of 16 Afghan villagers.
Karzai questioned whether only one US soldier could have been responsible for a massacre that took so many lives.
A series of blunders by the United States, including the killings in Kandahar province last Sunday and the inadvertent burning of copies of the Koran at a NATO base last month, has further strained already tense relations between the countries.
"This has been going on for too long. You have heard me before. It is by all means the end of the rope here," Karzai told reporters at the heavily fortified presidential palace.