The aircraft in question was identified as an Antonov AN-30, which repeatedly flew back and forth over the At-Tanf garrison and its surrounding area.
NATO members Denmark and the Netherlands have been leading international efforts to train pilots as well as support staff, maintain aircraft and ultimately enable the supply of F-16s to Ukraine.
“We urge Russian forces in Syria to cease this reckless behavior and adhere to the standards of behavior expected of a professional air force," said Air Force Lt. Gen. Alex Grynkewich.
Researchers at MIT have developed new algorithms that could help human pilots deal with extreme near-crash events.
The F-35 is the fifth-generation aircraft considered the best in the world and is the tip of the spear for both the US and Israel.
The Su-34 is Russia's premier fighter-bomber aircraft, but its fleet's ranks have reportedly been thinned out since the start of the Ukraine-Russia War.
The reason for lack of response from the pilots remains unclear to authorities.
US President Joe Biden told G7 leaders on Friday that Washington supports joint allied training programs for Ukrainian pilots on F-16s, senior US officials said.
Training on the US-made jets will take place in Europe and will require months to complete.
The US aircraft involved in the incident included F-16 and F-22 fighter jets, KC-135 stratotankers and E-3 AWACS, according to the statement.